The SETUP: Step by Step

by Dr. Tonny Mulder & Meike Mulder



Choose where to start, or scroll down:


STEP 1: The Component Checklist

First things first, you need to check if all the necessary components are present. Do this by using the checklist below:

ALSO: Read the descriptions carefully to get a feel of what every component does.

Setup Overview




GREAT! Proceed to the next STEP!
Please ask a student assistent!



STEP 2: Connecting The Components

Now it is time to connect all the components. For this, carefully follow these steps:


Every PsyPhy EEG setup has its own numberered case. All its components have the same number. Keep them together.

1. Place the Powerbank in the EEG amplifier and connect the Amplifier USB to the Powerbank. During the practical you find the powerbank in one of the loading docks in the room.

2. Insert one end of the Optical cable into the back of EEG amplifier and the other end into the USB to USB connectorbox.

3. Insert the LED side of the Serial cable into the PsyPhy EEG computer and the other end into the stimulus computer (your own computer).

4. Insert the cable of the Electrode 'adaptor' box into the front of the EEG amplifier. Be careful when removing this cable later. Press in the sides before pulling!

5. Power UP the PsyPhy EEG amplifier with the switch under the lid. The Red light should be flashing.  (RED = ON)

6. Power Up your PsyPhy EEG laptop. Get the password from the teaching staff.

7. Find the Name and IP address of YOUR EEG amplifier. It is posted on the inside of the Amplifier lid

8. Find the WIFI networks of your PsyPhy EEG laptop and select the one that is send by YOUR EEG amplifier. When connected, it should say: "No internet, secured ". The light in the amplifier should turn green (GREEN = connected), while the Red light remains flashing.

9. The PsyPhy EEG laptop is now ready to receive input from the EEG amplifier.

NOTE: Make sure the PsyPhy EEG laptop does not automatically (re)connect to eduroam or another Wi-Fi network during the recording. Do this by turning off "Connect automatically" for all other Wi-Fi networks.



Your PsyPhy EEG setup in the BOX 

Amplifier in box

1. Powerbank in EEG amplifier.

PsyPhy connect optical

2. Optical cable from USB to USB connectorbox to back of the EEG amplifier.

EEG laptop connect serial

3. USB Serial cable (LED ON) connected to PsyPhy EEG laptop     .

PsyPhy connect electrode box

4. Electrode 'adapter' box to EEG amplifier.

Amplifier label

7. Name, IP address and password EEG amplifier. Can be found inside the lid of the EEG amplifier

PsyPhy connect electrode box

8. WIFI settings on PsyPhy EEG laptop. Make sure to select YOUR PsyPhy_Amp. As you can see, two are available!!

Your set up should now look somewhat like this (minus the electrodes and cap):

Overview setup


NOTE: Please double check that the cables are inserted correctly and your PsyPhy EEG laptop is connected to the correct EEG amplifier!

NOTE 2: Make sure you understand this set-up before proceeding. For help, refer back to the explanations given in the Component Checklist!





STEP 3: Choosing Electrode Locations

The 10-20 System

The different placements of the electrodes on the cap are based on the International "10-20 system" for EEG recording. For a detailed explanation, click on the right!


Cap fit topview

The electrodes and the EEG Cap are placed according to the international standard. Important is the standardized location of the Cz electrode. See 4.1: Fitting the CAP

3.1 Base your electrodes on relevant literature:

You will have to decide where you want to measure. With a limited number of electrodes, you can't record all areas. So select your recording locations wisely.

Select literature that studied effects in a similar setting than yours and see where they placed the electrodes. Also, you can use the placements on which they found the strongest effect.

KEEP IN MIND: Be very precise on what kind of effect you are looking for: using visual stimuli does not necessarily mean the effect will be found over the visual cortex. For example, if you use words or pictures with different meanings in different context the effect will more likely take place over the temporal cortex. Also auditory ERPs are best recorded on PZ and are less effectively recorded on lateral positions. So: base this is on literature!

Spread your electrodes

The best signal is often measured at other locations than directly over the brain area where it is generated. As it passes through the skull and scalp it can be smeared out. The result of this is that EEG has low spatial selectivity (meaning it is hard to determine where the measured signal comes from exactly). On the upside, this also means that, even if you place an electrode next to where the maximum effect actually is, you will still pick it up. Consequently, placing two electrodes right next to each other does not give you much more information. Keep this in mind as well.

For a detailed explanation on the function of the different electrodes please visit the

Channel map

The PsyPhy EEG Adapter: Connect your choosen electrodes!

3.2 Now it's time to set up the configuration file (.XML file)!

This file is needed for the PsyPhy EEG recorder software to know which channels you want to measure and to setup the hardware connections.

READ CAREFULLY and Follow instructions!

1. Download the Default Configuration-file from Canvas, copy this on a USB stick and transfer it to the PsyPhy EEG computer. There, save it on the desktop.

IMPORTANT: Create a DUPLICATE and RENAME this file under a UNIQUE name, preferably containing the name of your group (e.g. PsyPhyC1.xml). This will be the configuration-file you can edit and the version that you will use throughout the EEG practical.


2. Next, download and read the PsyPhy Config file (XML).docx from Canvas AND the points below to determine what information you can/must change in the configuration-file and what information to leave unchanged:

3. Double click the XML file. It opens in Notepad!

  • Sample speed: Set sample_speed to 500.
  • 4. BEWARE: For now, LEAVE the channel settings as is.

    5. Scroll all the way down to the last code section called "hardware".

  • Network: This should match with the NAME of YOUR PsyPhy EEG-amplifier.
  • IP-adress: Check the IP-adress, this should match with the IP-adress on the PsyPhy EEG-amplifier.
  • COM-port number: To receive the markers from the stimulus laptop, you must set the correct COM-port number under the heading Hardware. You can find the COM-port number in the device manager of your PsyPhy EEG laptop (windows-button, type device manager, click on PORTS).
  • Channel map

    6. Now its time to select the channels you would like to record!

  • Channels: Determine which channels you want to record. The ones you decided to record in section 3.1.
  • Disable the channels that you do not measure by putting them in comments (<!-- Code to comment -->) see description of configuration file).

    8. Now you are ready for your recording!


    STEP 4: Fitting the CAP



    Select the cap size that fits your subject. 

    The green cap has a medium fit (56 cm) and fits most people. The light red cap is the largest (60 cm), and the dark red cap is the smallest (52 cm). The cap should be tight enough so the electrodes are close to the scalp but still comfortable for the subject to wear. Let the subject test out the caps.

    After a cap is chosen, let the participant take off the cap, and insert the electrodes on the locations chosen by you. Then let the participant put on the cap with electrodes.

    Orienting the cap

    Measure the head size of the subject from nasion to inion, and from the left tip of the ear to the tip of the right ear. The location of the Cz electrode should be in the center of these measures. Move the cap around if necessary and make sure it fits tight and symmetrical.

    Let the subject place the chin band.

    Now you are ready to gel the electrodes.


    gelling gellingref

    Gelling the electrodes

    1: Fill the curved syringe with electrode gel.

    2: Place the tip of the syringe through the electrode on the skull. Ask the subject whether    the tip is feld.

    3: Apply the gel while retracting the syringe.

    4: The reference and bias electrodes (red and blue earclips) are gelled by appying a drop of gel on the metal electrode disk and placing them on each of the earlobes.

    THE EEG_recorder Software

    STEP 5: Recording EEG

    Opening the EEG recorder software

    1. Open the recording software: 'FysioRecorder' on the PsyPhy desktop.

    2. Then open YOUR own configuration-file via the 'data acquisition' menu.

    desktop apps

    3. It should load pretty quickly and look like this:

    recorder overview

    4. Check if your choosen channels are going to be recorded.

  • Do this by checking the "Selected Channels" on the left of the screen. If these do not correspond to the locations you want to measure, then something is wrong with your configuration-file!
  • Starting a recording

    3. Start a recording by pressing "Start Block" on the bottom left.

  • A pop-up appears, saying no output-file was selected, and if you want to create a new one. Press "Yes".
  • Choose an informative name: this will be the name for the EEG data for this participant!

    The recording has now started! The  light on the amplifier should turn yellow (YELLOW = sending data). Give the signal a moment to stabilise. Be patient.

    KEEP IN MIND: you can NOT pause the recording by pressing "Stop Block". This will automatically save the data and CLOSE the software! From this point on, you can leave the recording running until the participant has completed the experiment.

    No output assigned pop-up


    4. Check whether the signal quality is good enough.

    4.1 Impedance Check:

  • Press Options > Impedance Check.
  • If the location of the electrode is red, the signal is poor quality. Manipulate the electrode and the amount of gel until all connected locations turn green. TIP: also check if the BIAS electrode is gelled properly. This is needed for the impedance check to work!

    4.2 Visibility of common artifacts/signals:

  • Muscle activity: Ask the participant to clench their jaw. This should be clearly visible and a factor 2/3x larger than brain activity.
  • Eye blinks: Ask the participant to repeatedly blink their eyes. This should be visible on the frontal electrodes (if recorded) and be about a factor 2 larger than brain activity.

    5. Once every channels shows a good signal, the participant can start the experiment on the stimulus laptop.

  • Check during the experiment if the timing of the markers corresponds with the experiment, e.g. when a picture is shown, or the participant presses a key.
  • Markers are shown as sudden spikes in the lowest channel (the marker channel), and the assigned number of the latest marker is shown in the bottom bar (see below)


    Impedance good


    Impedance bad

    Stopping a recording

    Once the participant has completed the whole experiment, the EEG recording can be stopped and saved:

    6. To stop the recording, press "Stop Block" in the bottom left. The software stops automatically and the file is saved!

  • The EEG data will be a .mat file saved under the (informative) name you entered when creating the output file at the beginning. You can find this file in the "DATA" folder on the desktop.

  • Cleaning up after recording

    When a recording is finished, always complete these steps right after:

    1. First, remove the reference & bias, then remove the cap with the rest of the electrodes.

    2. Remove as much gel from the hair of the participant as possible using paper tissue or a towel.

    3. Gently rinse the electrodes clean with warm water. IMPORTANT: the electrodes themselves can get wet, however keep the plugs (which are plugged into the electrode box) DRY at all times!

  • If the gell does not come off by using just water: use a soft brush (e.g. toothbrush) to get the remaining gel off.
  • 4. Dry off the electrodes immediately after washing and double check if no gel remains on the electrode. When old gel dries up on the electrode, it can block the signal during the next recording.

    5. Clean the syringe with water and dry it off with paper tissue.

    6. Clean the cap by removing gel rests with a bit of tissue. If possible: when a lot of gel has gotten on the cap, wash it out with warm water by hand, and leave it out to dry before measuring the next participant.


    Cap fit

    You have now succesfully recorded EEG!

    For ERP analysis, go to:

    For Oscillation analysis, go to:



    Trouble Shooting

    When Things go not completely as planned

    Will be uploaded soon